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Gas Chromatography Tandem Mass Spectrometry (GC-MSMS): Principles and Applications

Tues 2 July to Wed 3 July Or Tues 16 July to Wed 17 July
Workshop Code (C7)

Gas chromatography tandem mass spectrometry (GC-MSMS): Principles and Applications

This course offers comprehensive knowledge about the GC-MS instrument, including its theory and how to use its accessories. GC-MS is a powerful tool for detecting organic pollutants in the environment, providing significant and reliable results. The lab sessions cover various aspects such as mass spectrometry instrumentation, tuning, mass calibration, the impact of different parameters on data acquisition methods (Full Scan and/or SIM), and an introduction to interpreting mass spectra. The course also includes qualitative and quantitative analyses.

Day-1

  • 9:30 AM – 11:00 AM: Introduction to GC-MSMS; Principles of Mass Spectroscopy, Sample introduction (injection techniques), Scan modes, Ionization techniques
  • 11:00 AM – 12:30 PM: Introduction to GC-MSMS; Principles of Mass Spectroscopy, Sample introduction (injection techniques), Scan modes, Ionization techniques
  • 12:30 PM – 1:00 PM: Break
  • 1:00 PM – 4:00 PM: Hands-on activity: GC components, MSMS components, Tuning and Calibration, Calibration report generation, MS Tune File, Explanation of MS Method and GC Method, setting up a batch, setting up acquisition method, injection of standard solution

Day-2 

  • 9:30 AM – 11:00 AM: GC-MS/MS System (Sequencing, MRM Methods Development, Calibration, Reporting, Troubleshooting, Maintenance)
  • 11:00 AM – 12:30 PM: GC-MS/MS System (Sequencing, MRM Methods Development, Calibration, Reporting, Troubleshooting, Maintenance)
  • 12:30 PM – 1:00 PM: Break
  • 1:00 PM – 4:00 PM: Introduction to Quantitative software, Qualitative software, and integration –  Maintaining the GC-MS instrument and troubleshooting the problems related to gas supply, inlet, auto-sampler, column, and detector

For more inquiries about the workshop, kindly contact:

Ibrahim.badr@gu.edu.eg